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Thomas Kleyman (PI)
University of Pittsburgh
Proposed studies will determine specific functional properties of tubular epithelia in human kidney organoids. Key transporters, cytoskeletal elements, and other relevant proteins will be localized in epithelia within developing kidney organoids. Key physiological properties of epithelia within distinct tubular segments in organoids will be defined. The uptake of fluorescent albumin and dextran in proximal tubules will be examined to demonstrate cell specific receptor-mediated and/or fluid phase endocytosis. H+ secretion by specific epithelial H+ transporters in specific cells and tubular segments will be determined. Functional expression of K+ and Na+ channels in specific cells and tubular segments will be determined. Na+ and K+ transport in isolated, microperfused tubules will be determined.